Hipoxia modulates the secretion of growth factors in of human umbilical cord-derived mesenchymal stem cells

Background: Mesenchymal stem cell (MSC) has great potential as therapies due its ability to regenerate tissue damage and promote homeostasis. Preconditioning of MSC in low oxygen concentration been shown affect the therapeutic these cells. This study aimed compare characteristic secretion trophic factors MSCs cultured under hypoxia normoxia. Methods: were isolated from Wharton's jelly human umbilical cord (UC) by explant method characterized flow cytometry. Following 24 hours CoCl2induced hypoxic culture, viability metabolic activity analyzed trypan blue exclusion test methyl thiazolyl tetrazolium (MTT) assay, respectively. The hepatocyte growth factor (HGF) vascular endothelial (VEGF) was assessed conditioned medium using enzyme-linked immunosorbent assay (ELISA) method. Results: Flow cytometry analysis showed >99% population cells positive for CD73 CD90 >62% CD105. While not affected condition, rate decreased conditioning. In line with reduced activity, UC-derived produced less HGF than normoxic counterpart. Compared MSC, preconditioned secreted higher level VEGF (p